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Alomone Labs News & Updates
 
 
 
 
 

News & Updates - September 2016

 

Products for Immuno-Colocalization

Alomone Labs has developed three innovative product lines for studying protein-protein interaction and/or protein co-localization:

Primary antibodies conjugated to biotin or ATTO-fluorescent dyes: These antibodies can be used in immunohistochemistry (IH) and immunocytochemistry (IC) with same species antibodies.

Antibodies raised in guinea pig: These second species antibodies can be used with any other non-guinea pig second species antibodies in immuno-colocalization studies such as IH and IC.

Biotinylated neurotrophins: These labeled neurotrophins can be used with any antibody to obtain highly specific localization.

Click here to view our complete line of immuno-colocalization products.

Anti-Vesicular GABA Transporter-ATTO-594

AGT-005-AR

Immunohistochemical staining of rat frozen cerebellum sections: Anti-Vesicular GABA Transporter-ATTO-594 antibody (#AGT-005-AR), (1:100). VGAT staining (red) is most intensely expressed in the granule and pyramidal layer.

Anti-Kir4.1

AGP-012

Immunohistochemical staining of rat frozen brain sections: Anti-Kir4.1 guinea pig antibody (#AGP-012), (1:300). Kir4.1 staining (green) reveals Kir4.1 clusters in the molecular layer. GFAP staining is shown in red.

Anti-Vesicular Monoamine Transporter 2-ATTO-488

AMT-006-AG

Immuno-colocalization of VMAT2 and TRPC3 in rat brain

Immunohistochemical staining of perfusion-fixed frozen rat substancia nigra sections using Anti-Vesicular Monoamine Transporter 2-ATTO-488 antibody (#AMT-006-AG), (1:60), (green) and Anti-TRPC3-ATTO-594 antibody (#ACC-016-AR), (1:60), (red). Ubiquitous colocalization of VMAT2 and TRPC3 in cells of the substantia nigra pars compacta is observed.

Products for Live Cell Imaging (LCI)

Alomone Labs is committed to developing cutting edge reagents for visualizing neurotrophin receptors in live cell imaging (LCI) experiments. For this purpose we have established a line of antibodies recognizing extracellular epitopes of various receptors. Below are new antibodies ideal for LCI.

Click here to view our complete line of LCI products.

Anti-NBCe1 (extracellular)

ANT-075

Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells: Extracellular staining of cells with Anti-NBCe1 (extracellular) antibody (#ANT-075), (1:100), (red) merged with live view of cells.

Anti-TRPM8 (extracellular)

ACC-049

Immunocytochemical staining of intact living rat root dorsal root ganglion (DRG) cells: Extracellular staining of cells with Anti-TRPM8 (extracellular) antibody (#ACC-049), (1:500), (red).

Anti-TRPC6 (extracellular)

ACC-120

Expression of TRPC6 in rat PC12 cells

Immunocytochemical staining of intact living PC12 cells. Extracellular staining of cells with Anti-TRPC6 (extracellular) antibody (#ACC-120), (1:50), (red).

New & Noteworthy

A Potent and Selective Blocker of NaV1.6 Channel!

4,9-Anhydrotetrodotoxin (#T-560) is a specific and extremely potent NaV1.6 channel blocker, with an IC50 of 7.8 nM.

T-560

Alomone Labs 4,9-Anhydrotetrodotoxin selectively blocks NaV1.6 channels expressed in Xenopus oocytes.

A. Time course of 4,9-Anhydrotetrodotoxin (#T-560) inhibition of NaV1.6 maximum current amplitude. Membrane potential was held at -100 mV and currents were elicited by a 100 ms voltage step to 0 mV every 10 sec. 4,9-Anhydrotetrodotoxin applied at 10 nM and 50 nM, as indicated (bars), reversibly inhibits NaV1.6 currents in a concentration dependent manner. B. Superimposed traces of NaV1.6 current upon application of control, and of 10 nM and 50 nM 4,9-Anhydrotetrodotoxin (as indicated), taken from the recording shown in A.

Ion Channel Products in Featured Papers

KCa3.1 Modulates Placental Syncytialization

IKCa (KCa3.1, SK4) channel is involved in the formation of syncytiotrophoblast and syncytiotrophoblast volume regulation. Immunocytochemical staining of isolated human cytotrophoblasts using mouse monoclonal Anti-KCa3.1 (SK4) (extracellular) antibody (#ALM-051) showed that SK4 channel is indeed expressed in these cells (Diaz, P. et al. (2014) PLoS ONE 9, e90961.).

ALM-051

Expression of KCa3.1 channel in human cytotrophoblasts
Immunocytochemical staining of human cytotrophoblast using mouse monoclonal Anti-KCa3.1 (SK4) (extracellular) antibody (#ALM-051). A. Methanol fixed cells (15 hrs). B. Methanol fixed cells (66 hrs).

Adapted from Diaz, P. et al. (2014) with kind permission of Dr. Diaz, P. and Dr. Greenwood, S.L. from Maternal and Fetal Health Research Centre, Institute of Human Development University of Manchester, Manchester, United Kingdom and St. Mary’s Hospital, Central Manchester University Hospitals National Health Service (NHS) Foundation Trust, Manchester Academic Health Science Centre, Manchester, United Kingdom.

Modulator Magazine

The Modulator is dedicated to hot topics and recent developments in ion channel research. In each issue, we present elegant and exciting new ways our products are being used in papers from reputable journals. 

Take a look at our latest Modulator issue.

Subscribe and receive a free printed copy!

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